The enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-PheGln-↓-Gly-Pro (LEVLFQ↓GP). The enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃.
SUMO proteins are a family of small proteins covalently attached to other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in many cellular process